Anti-Human CREB1 Antibody [K40043_5E11]

IHC-P analysis of human testis tissue by anti-human CREB1 antibody (K40043_5E11). 

IHC-P was performed using sections of the formalin-fixed paraffin-embedded human testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with anti-human CREB1 primary antibody (K40043_5E11) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. 

Result: Cells in seminiferous ducts are positively stained at the nuclei.