IHC-P analysis of human tonsil by anti-human CLSPN antibody (K52026_11H4).
IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human CLSPN primary antibody (K52026_11H4) at 2 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Squamous epithelial cells,non-germinal center cells and germinal center cells are positively stained at the nuclei.
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Anti-Human CLSPN Antibody [K52026_11H4]
Host / isotype
Mouse / IgG2b
Recombinant human CLSPN
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)