100 ng of recombinant human IgG was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K03018_3F3 at 5 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the secondary antibody. Human IgG band was visualized using ECL Western Blotting Substrate.
Result: K03018_3F3 can detect human IgG by Western blotting.
IHC-P analysis of human tonsil tissue by anti-human IgG antibody (K03018_3F3).
IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgG primary antibody (K03018_3F3) at 2 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG (which cross reacts with Rat IgG) was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin.
Result: Germinal center cells, non-germinal center cells and squamous epithelial cells are positively stained at the cytoplasm (Only positively stained Germinal center cells and non- germinal center cells are shown here).
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Anti-Human IgG Antibody [K03018_3F3]
Host / isotype
Rat / IgG2a
Kinetic characterization by BLI (biolayer interferometry)
Protein G purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.