Anti-Human IgE Antibody [1A2]
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Sandwich ELISA
Microtiter wells were coated with 4C3 at 3 µg/mL as the capture antibody. Human IgE was used as the antigen. Peroxidase conjugated mouse anti-human IgE monoclonal antibody (1A2) was used as the detection antibody.
Result: 1A2 and 4C3 can be used as a matched antibody pair to detect and quantify the concentration of human IgE.
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Full details
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Product name
Anti-Human IgE Antibody[1A2]
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Antibody specificity
Ig epsilon chain C region (IgE)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
1A2
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Host / isotype
Mouse / IgG1
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Immunogen
Recombinant human IgE
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Cross reactivity
Not tested
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.01% preservative
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Conjugation
Conjugated with peroxidase
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Sandwich ELISA
1 µg/mL
Peroxidase conjugated 1A2 can pair with 4C3 for sandwich ELISA. 4C3 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Immunoglobulin heavy constant epsilon
Gene name:
IGHE
Uniprot Accession:
P01854
Organism:
Homo sapiens (Human)
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Paired antibody information
1A2 may pair with 4C3 for sandwich based immune assays.
