Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT150 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Rat IgG1 band was visualized using ECL Western Blotting Substrate.
Lane 1: 100 ng of Rat IgG1
Lane 2: 100 ng of Rat IgG2a
Lane 3: 100 ng of Rat IgG2b
Lane 4: 100 ng of Rat IgG2c
Lane 5: 100 ng of Rat IgM
Result: KT150 can detect rat IgG1 by Western blotting.
There are no related products
Anti-Rat IgG2a Antibody [KT150]
Host / isotype
Mouse / IgG1
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
g gamma-2A chain C region
Rattus norvegicus (Rat)