Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K06263_3B5 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgE band was visualized using ECL Western Blotting Substrate.
Lane 1: 100 ng of human IgA
Lane 2: 100 ng of human IgG
Lane 3: 100 ng of human IgE
Result: K06263_3B5 can detect human IgE by Western blotting.
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Anti-Human IgE Antibody [K06263_3B5]
Host / isotype
Mouse / IgG1
Recombinant human IgE
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Immunoglobulin heavy constant epsilon
Homo sapiens (Human)