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Anti-Human IgG2 Antibody [K06306_22C5]

Catalog No. K06306C22C05C
  • Immunohistochemistry

    IHC-P analysis in human tonsil tissue by anti-human IgG2 antibody (K06306_22C5). 

    IHC-P was performed using sections of formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min.Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgG2 primary antibody (K06306_22C5) at 2 µg/mL at room temperature for 1 h.Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. 

    Results: Germinal center cells and squamous epithelial cells are positively stained at cytoplasm (Only cells stained at germinal center were shown here).

  • Cross reactivity

    Microtiter wells were coated with human immunoglobulins with various isotypes. K06302_22C5 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. 

    Result: K06302_22C5 does not cross-react with human IgG1, IgG3, IgG4, IgA, IgM and IgE.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IgG2 Antibody [K06306_22C5]

  • Antibody specificity

    Human IgG2

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG2b

  • Immunogen

    Recombinant human IgG2

  • Cross reactivity

    K06306_22C5 does not cross-react with human IgG1, IgG3, IgG4, IgA, IgE and IgM

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Purification

    Protein A purified from cell culture supernatants

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C


Full details

Recommend concentration


  • Indirect ELISA

    1 µg/mL

  • Immunohistochemistry-paraffin (IHC-P)

    2 µg/mL


The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Immunoglobulin heavy constant gamma 2

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)