Human IgA, human IgM, human IgG and human IgE were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT41 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgA band was visualized using ECL Western Blotting Substrate.
Lane 1: 100 ng of human IgA
Lane 2: 100 ng of human IgM
Lane 3: 100 ng of human IgG
Lane 4: 100 ng of human IgE
Result: KT41 can specifically detect human IgA by Western blotting.
Microtiter wells were coated with KT41 at 5 µg/mL as the capture antibody. Human IgA was used as the antigen. Peroxidase conjugated mouse anti-human IgA monoclonal antibody (KT40) was used as the detection antibody.
Result: KT41 and KT40 can be used as a matched antibody pair to detect and quantify the concentration of human IgA.
IHC-P analysis of human tonsil tissue by anti-human IgA antibody (KT41).
IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgA primary antibody (KT41) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with secondary antibody was used as background control.
Result: Germinal center cells and squamous epithelial cells are positively stained at the cytoplasm.
Microtiter wells were coated with human immunoglobulins with various isotypes. KT41 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: KT41 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgM and IgE.
There are no related products
Anti-Human IgA Antibody [KT41]
Ig alpha-1 chain C region and Ig alpha-2 chain C region (IgA1, IgA2)
Host / isotype
Mouse / IgG1
Recombinant human IgA2
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
KT41 can pair with peroxidase conjugated KT40 for sandwich ELISA. KT41 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Immunoglobulin heavy constant alpha 2 (A2m marker)
Homo sapiens (Human)
Paired antibody information
KT41 may pair with KT40 for sandwich based immune assays.