Anti-Human IgA Antibody [KT41]

Human IgA, human IgM, human IgG and human IgE were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT41 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgA band was visualized using ECL Western Blotting Substrate.

Lane 1: 100 ng of human IgA

Lane 2: 100 ng of human IgM

Lane 3: 100 ng of human IgG

Lane 4: 100 ng of human IgE

Result: KT41 can specifically detect human IgA by Western blotting.

Microtiter wells were coated with KT41 at 5 µg/mL as the capture antibody. Human IgA was used as the antigen. Peroxidase conjugated mouse anti-human IgA monoclonal antibody (KT40) was used as the detection antibody. 

Result: KT41 and KT40 can be used as a matched antibody pair to detect and quantify the concentration of human IgA. 

IHC-P analysis of human tonsil tissue by anti-human IgA antibody (KT41).

IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgA primary antibody (KT41) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with secondary antibody was used as background control.

Result: Germinal center cells and squamous epithelial cells are positively stained at the cytoplasm.

Microtiter wells were coated with human immunoglobulins with various isotypes. KT41 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.

Result: KT41 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgM and IgE.