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Anti-Human IgE Antibody [4C3]

Catalog No. K00001M04C03C
  • Sandwich ELISA

    Microtiter wells were coated with 4C3 at 3 µg/mL as the capture antibody. Human IgE was used as the antigen. Peroxidase conjugated mouse anti-human IgE monoclonal antibody (1A2) was used as the detection antibody. 

    Result: 4C3 and 1A2 can be used as a matched antibody pair to detect and quantify the concentration of human IgE.

  • Cross reactivity

    Microtiter wells were coated with human immunoglobulins with various isotypes. 4C3 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

    Result: 4C3 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgA and IgM.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IgE Antibody [4C3]

  • Antibody specificity

    Ig epsilon chain C region (IgE)

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG1

  • Immunogen

    Recombinant human IgE

  • Cross reactivity

    4C3 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgA and IgM.

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Purification

    Protein A purified from cell culture supernatants

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C


Full details

Recommend concentration


  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    4C3 can pair with peroxidase conjugated 1A2 for sandwich ELISA. 4C3 is used as the capture antibody.


The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Immunoglobulin heavy constant epsilon

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    4C3 may pair with 1A2 for sandwich based immune assays.