Anti-Human IgE Antibody [4C3]
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Sandwich ELISA
Microtiter wells were coated with 4C3 at 3 µg/mL as the capture antibody. Human IgE was used as the antigen. Peroxidase conjugated mouse anti-human IgE monoclonal antibody (1A2) was used as the detection antibody.
Result: 4C3 and 1A2 can be used as a matched antibody pair to detect and quantify the concentration of human IgE.
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Cross reactivity
Microtiter wells were coated with human immunoglobulins with various isotypes. 4C3 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: 4C3 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgA and IgM.
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Full details
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Product name
Anti-Human IgE Antibody [4C3]
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Antibody specificity
Ig epsilon chain C region (IgE)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
4C3
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Host / isotype
Mouse / IgG1
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Immunogen
Recombinant human IgE
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Cross reactivity
4C3 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgA and IgM.
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Kinetic characterization by BLI (biolayer interferometry)
Not tested
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Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
4C3 can pair with peroxidase conjugated 1A2 for sandwich ELISA. 4C3 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Immunoglobulin heavy constant epsilon
Gene name:
IGHE
Uniprot Accession:
P01854
Organism:
Homo sapiens (Human)
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Paired antibody information
4C3 may pair with 1A2 for sandwich based immune assays.
