Anti-Human IgA Antibody [KT153]

Human IgM, human IgG, human IgE and human IgA were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT153 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgA band was visualized using ECL Western Blotting Substrate.

Lane 1: 100 ng of human IgM 

Lane 2: 100 ng of human IgG 

Lane 3: 100 ng of human IgE 

Lane 4: 100 ng of human IgA 

Result: KT153 can specifically detect human IgA by Western blotting.

IHC-P analysis of human tonsil tissue by anti-human IgA antibody (KT153).

Immunohistochemistry was performed using sections of the formalin-fixed paraffin- embedded human tonsil. Antigen retrieval was done using boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Removal of endogenous peroxidase activity was done by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgA primary antibody (KT153) at 2 µg/mL at room temperature for 1 h and poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin.

Result: A subset of cells are positively stained at cytoplasm.

Microtiter wells were coated with human immunoglobulins with various isotypes. KT153 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.

Result: KT153 reacts with human IgA1 and IgA2. KT153 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgM and IgE.