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Anti-Human IgG4 Antibody [KT209]

Catalog No. K06216M03D08C
  • Western blotting

    Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT209 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. KT209 band was visualized using ECL Western Blotting Substrate. 

    Lane 1: 100 ng of Human IgG1 

    Lane 2: 100 ng of Human IgG2 

    Lane 3: 100 ng of Human IgG3 

    Lane 4: 100 ng of Human IgG4 

    Lane 5: 100 ng of Human IgA 

    Lane 6: 100 ng of Human IgE 

    Lane 7: 100 ng of Human IgM 

    Result: KT209 can detect human IgG4 by Western blotting.


  • Sandwich ELISA

    Microtiter wells were coated with KT209 at 5 µg/mL as the capture antibody. Human IgG4 was used as the antigen. Peroxidase conjugated mouse anti-human IgG4 monoclonal antibody (KT131) was used as the detection antibody. 

    Result: KT209 and KT131 can be used as a matched antibody pair to detect and quantify the concentration of human IgG4.


Associated Products
  • There are no related products

Overview

Full details

  • Product name

    Anti-Human IgG4 Antibody [KT209]

  • Antibody specificity

    Human IgG4

  • Species reactivity

    Human

  • Clonality

    Monoclonal

  • Clone number

    KT209

  • Host / isotype

    Mouse / IgG1

  • Immunogen

    Recombinant human IgG4

  • Cross reactivity

    Not tested

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Form

    Liquid

  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation

    Unconjugated

  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

Applications

Full details

Recommend concentration

Note

  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    5 µg/mL

    KT209 can pair with peroxidase conjugated KT131 for sandwich ELISA. KT209 is used as the capture antibody.

Note

The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Immunoglobulin heavy constant gamma 4

    • Gene name:

      IGHG4

    • Uniprot Accession:

      P01861

    • Organism:

      Homo sapiens (human)

  • Paired antibody information

    KT209 may pair with K06087_7D12 and KT131 for sandwich based immune assays.