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Anti-Human IgG3 Antibody [KT133]

Catalog No. K06089M09H08C
  • Western blotting

    Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT133 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG3 band was visualized using ECL Western Blotting Substrate. 

    Lane 1: 100 ng of rat IgG1 

    Lane 2: 100 ng of human IgE 

    Lane 3: 100 ng of human IgM 

    Lane 4: 100 ng of human IgA 

    Lane 5: 100 ng of human IgG4 

    Lane 6: 100 ng of human IgG3 

    Lane 7: 100 ng of human IgG2 

    Lane 8: 100 ng of human IgG1 

    Result: KT133 can specifically detect human IgG3 by Western blotting.

  • Sandwich ELISA

    Microtiter wells were coated with KT133 at 3 µg/mL as the capture antibody. Human IgG3 was used as the antigen. Peroxidase conjugated mouse anti-human IgG3 monoclonal antibody (K06089_8E4) was used as the detection antibody. 

    Result: KT133 and K06089_8E4 can be used as a matched antibody pair to detect and quantify the concentration of human IgG3.

  • Immunohistochemistry

    IHC-P analysis of human tonsil tissue by anti-human IgG3 antibody (KT133). 

    IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgG3 primary antibody (KT133) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. 

    Result: Germinal center cells and squamous epithelial cells are positively stained at cytoplasm and membranous.

  • Cross reactivity

    Microtiter wells were coated with human immunoglobulins with various isotypes. KT133 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

    Result: KT133 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IgG3 Antibody [KT133]

  • Antibody specificity

    Human IgG3

  • Species reactivity

    Human, Rat

  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG1

  • Immunogen

    Recombinant human IgG3

  • Cross reactivity

    Not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon): 4.33x 105 M-1s-1
    Dissociation rate constant (koff): 1.04x 10-4 s-1
    Equilibrium dissociation constant (KD):2.40x 10-10 M

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C


Full details

Recommend concentration


  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    KT133 can pair with peroxidase conjugated K06089_8E4 for sandwich ELISA. KT133 is used as the capture antibody.

  • Immunohistochemistry-paraffin (IHC-P)

    1 µg/mL


The applications above have already been verified. The antibody may be suitable for additional applications. 

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Immunoglobulin heavy constant gamma 3

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    KT133 may pair with K06089_8E4 for sandwich based immune assays.