Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT130 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG4 band was visualized using ECL Western Blotting Substrate.
Lane 1: 100 ng of human IgG1
Lane 2: 100 ng of human IgG2
Lane 3: 100 ng of human IgG3
Lane 4: 100 ng of human IgG4
Lane 5: 100 ng of human IgA
Lane 6: 100 ng of human IgM
Lane 7: 100 ng of human IgE
Result: KT130 can specifically detect human IgG4 by Western blotting.
Microtiter wells were coated with KT130 at 5 µg/mL as the capture antibody.Peroxidase conjugated mouse anti-human IgG4 monoclonal antibody (KT209) was used as the detection antibody.
Result: KT130 and KT209 can be used as a matched antibody pair to detect and quantify the concentration of human IgG4.
IHC-P analysis of human tonsil tissue by anti-human IgG4 antibody (KT130).
IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgG4 primary antibody (KT130) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin.
Result: Squamous epithelial cells and non-germinal center cells are positively stained at cytoplasm.
Microtiter wells were coated with human immunoglobulins with various isotypes. KT130 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: KT130 does not cross-react with human IgG1, IgG2, IgG3, IgA, IgM and IgE.
There are no related products
Anti-Human IgG4 Antibody [KT130]
Host / isotype
Mouse / IgG1
Recombinant human IgG4
Not cross-react with human IgG1, IgG2, IgG3, IgA, IgE and IgM.
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 3.98x 104 M-1s-1
Dissociation rate constant (koff):4.06x 10-4 s-1
Equilibrium dissociation constant (KD):1.02x 10-8 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
KT130 can pair with peroxidase conjugated KT209 for sandwich ELISA. KT130 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Immunoglobulin heavy constant gamma 4
Homo sapiens (Human)
Paired antibody information
KT130 may pair with KT209, K06087_3F8, K06087_4D3, K06216_5G4, K06087_10B2, K06087_13B6 and K06216_14H8 for sandwich based immune assays.