Your privacy is very important to us.When you visit our website,please agree to the use of all cookies.For more information about personal data processing,please go to Privacy.

Anti-Human IgG4 Antibody [KT130]

Catalog No. K06087M09G12C
  • Western blotting

    Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT130 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG4 band was visualized using ECL Western Blotting Substrate. 

    Lane 1: 100 ng of human IgG1 

    Lane 2: 100 ng of human IgG2 

    Lane 3: 100 ng of human IgG3 

    Lane 4: 100 ng of human IgG4 

    Lane 5: 100 ng of human IgA 

    Lane 6: 100 ng of human IgM 

    Lane 7: 100 ng of human IgE 

    Result: KT130 can specifically detect human IgG4 by Western blotting.

  • Sandwich ELISA

    Microtiter wells were coated with KT130 at 5 µg/mL as the capture antibody.Peroxidase conjugated mouse anti-human IgG4 monoclonal antibody (KT209) was used as the detection antibody. 

    Result: KT130 and KT209 can be used as a matched antibody pair to detect and quantify the concentration of human IgG4.

  • Immunohistochemistry

    IHC-P analysis of human tonsil tissue by anti-human IgG4 antibody (KT130). 

    IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgG4 primary antibody (KT130) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. 

    Result: Squamous epithelial cells and non-germinal center cells are positively stained at cytoplasm.

  • Cross reactivity

    Microtiter wells were coated with human immunoglobulins with various isotypes. KT130 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

    Result: KT130 does not cross-react with human IgG1, IgG2, IgG3, IgA, IgM and IgE.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IgG4 Antibody [KT130]

  • Antibody specificity

    Human IgG4

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG1

  • Immunogen

    Recombinant human IgG4

  • Cross-reactivity

    Not cross-react with human IgG1, IgG2, IgG3, IgA, IgE and IgM.

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon): 3.98x 104 M-1s-1
    Dissociation rate constant (koff):4.06x 10-4 s-1
    Equilibrium dissociation constant (KD):1.02x 10-8 M

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C


Full details

Recommend concentration


  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    5 µg/mL

    KT130 can pair with peroxidase conjugated KT209 for sandwich ELISA. KT130 is used as the capture antibody.

  • Immunohistochemistry-paraffin (IHC-P)

    1 µg/mL


The applications above have already been verified. The antibody may be suitable for additional applications. 

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Immunoglobulin heavy constant gamma 4

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    KT130 may pair with KT209, K06087_3F8, K06087_4D3, K06216_5G4, K06087_10B2, K06087_13B6 and K06216_14H8 for sandwich based immune assays.