Rat IgG1, rat IgG2a, rat IgG2b, rat IgG2c and rat IgM were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT99 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Rat IgG2b band was visualized using ECL Western Blotting Substrate.
Lane 1: 100 ng of rat IgG1
Lane 2: 100 ng of rat IgG2a
Lane 3: 100 ng of rat IgG2b
Lane 4: 100 ng of rat IgG2c
Lane 5: 100 ng of rat IgM
Result: KT99 can specifically detect rat IgG2b by Western blotting.
There are no related products
Anti-Rat IgG2c Antibody [KT99]
Host / isotype
Mouse / IgG1
Kinetic characterization by BLI (biolayer interferometry)
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Ig gamma-2C chain C region
Rattus norvegicus (Rat)