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Anti-Human IgM Antibody [KT194]

Catalog No. K00001M04C05C
  • Western blotting

    Various samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT194 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgM band was visualized using ECL Western Blotting Substrate.

    Lane 1: 100 ng of human IgA

    Lane 2: 100 ng of human IgE 

    Lane 3: 100 ng of human IgG

    Lane 4: 100 ng of human IgM

    Lane 5: 100 ng of mouse IgM 

    Result: KT194 can specifically detect human IgM by Western blotting.

  • Immunohistochemistry

    IHC-P analysis of human tonsil tissue by anti-human IgM antibody

    Immunohistochemistry was performed using sections of the formalin-fixed paraffin-embedded human tonsil. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Removal of endogenous peroxidase activity was done by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgM primary antibody (KT194) at 2 µg/mL at room temperature for 1 h and poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.

    Result: A few germinal center cells and squamous epithelial cells are positively stained at the cytoplasm.

  • Cross-reactivity

    Microtiter wells were coated with human immunoglobulins with various isotypes. KT194 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.

    Result: KT194 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgA and IgE.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IgM Antibody [KT194]

  • Antibody specificity

    Human IgM

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG1

  • Immunogen

    Recombinant human IgM

  • Cross-reactivity

    KT194 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgA and IgE.

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

  • Purification

    Protein A purified from cell culture supernatants


Full details

Recommend concentration


  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Immunohistochemistry-paraffin (IHC-P)

    2 µg/mL


The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Immunoglobulin heavy constant mu

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)