Anti-Human IgM Antibody [KT16]

Human IgG, human IgA, human IgM and human IgE were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT16 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgM band was visualized using ECL Western Blotting Substrate. 

Lane 1: 100 ng of human IgG 

Lane 2: 100 ng of human IgA 

Lane 3: 100 ng of human IgM 

Lane 4: 100 ng of human IgE 

Result: KT16 can specifically detect human IgM by Western blotting.

Microtiter wells were coated with KT16 at 5 µg/mL as the capture antibody. Human IgM was used as the antigen. Peroxidase conjugated mouse anti-human IgM monoclonal antibody (KT38) was used as the detection antibody. 

Result: KT16 and KT38 can be used as a matched antibody pair to detect and quantify the concentration of human IgM.

Microtiter wells were coated with human immunoglobulins with various isotypes. KT16 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

Result: KT16 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgA and IgE