IHC-P analysis of human tonsil tissue by anti-human IGKC antibody.
Immunohistochemistry was performed using sections of the formalin-fixed paraffin- embedded human tonsil. Antigen retrieval was done using boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Removal of endogenous peroxidase activity was done by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IGKC primary antibody (KT171) at 1 µg/mL at room temperature for 1 h and poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Germinal center cells, non-germinal center cells and squamous epithelial cells are positively stained at the cytoplasm.
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Anti-Human IGKC Antibody [KT171]
Human kappa light chain protein (Bence Jones protein)
Host / isotype
Mouse / IgG1
Native human kappa light chain protein
Kinetic characterization by BLI (biolayer interferometry)
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Immunoglobulin kappa constant
Homo sapiens (Human)