Microtiter wells were coated with K06089_13E12 at 3 µg/mL as the capture antibody. Recombinant human IgG3 (Cat. KAA001H01C03C) was used as the antigen. Peroxidase conjugated mouse anti-human IgG3 monoclonal antibody (K16244_1A5) was used as the detection antibody.
Result: K06089_13E12 and K16244_1A5 can be used as a matched antibody pair to detect and quantify the concentration of human IgG3.
Microtiter wells were coated with human immunoglobulins with various isotypes. K06089_13E12 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K06089_13E12 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.
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Anti-Human IgG3 Antibody [K06089_13E12]
Host / isotype
Mouse / IgG2a
Recombinant human IgG3
K06089_13E12 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon):2.08x 105 M-1s-1
Dissociation rate constant (koff): 5.12x 10-4 s-1
Equilibrium dissociation constant (KD):2.46x 10-9 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
K06089_13E12 can pair with peroxidase conjugated K16244_1A5 for sandwich ELISA. K06089_13E12 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Immunoglobulin heavy constant gamma 3
Homo sapiens (Human)
Paired antibody information
K06089_13E12 may pair with K16244_1A5, K06089_8E4, K16244_8D9 and K16244_1A5 for sandwich based immune assays.