Various samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K16244_4E11 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG3 band was visualized using ECL Western Blotting Substrate.
Lane 1: 100 ng of human IgG1
Lane 2: 100 ng of human IgG2
Lane 3: 100 ng of human IgG3
Lane 4: 100 ng of human IgG4
Lane 5: 100 ng of human IgA
Lane 6: 100 ng of human IgM
Lane 7: 100 ng of human IgE
Lane 8: 100 ng of rat IgG1
Result: K16244_4E11 can specifically detect human IgG3 by Western blotting.
Microtiter wells were coated with human immunoglobulins with various isotypes. K16244_4E11 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K16244_4E11 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.
There are no related products
Anti-Human IgG3 Antibody [K16244_4E11]
Host / isotype
Mouse / IgG1
Recombinant human IgG3
K16244_4E11 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.
Kinetic characterization by BLI (biolayer interferometry)
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Immunoglobulin heavy constant gamma 3
Homo sapiens (Human)