Anti-Human IgG3 Antibody [K16244_4E11]

Various samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K16244_4E11 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG3 band was visualized using ECL Western Blotting Substrate.

Lane 1: 100 ng of human IgG1

Lane 2: 100 ng of human IgG2

Lane 3: 100 ng of human IgG3

Lane 4: 100 ng of human IgG4

Lane 5: 100 ng of human IgA

Lane 6: 100 ng of human IgM

Lane 7: 100 ng of human IgE

Lane 8: 100 ng of rat IgG1

Result: K16244_4E11 can specifically detect human IgG3 by Western blotting.

Microtiter wells were coated with human immunoglobulins with various isotypes. K16244_4E11 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.

Result: K16244_4E11 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.