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Anti-Human IgG3 Antibody [K16244_4E11]

Catalog No. K16244U04E11C
  • Western blotting

    Various samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K16244_4E11 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG3 band was visualized using ECL Western Blotting Substrate.

    Lane 1: 100 ng of human IgG1

    Lane 2: 100 ng of human IgG2

    Lane 3: 100 ng of human IgG3

    Lane 4: 100 ng of human IgG4

    Lane 5: 100 ng of human IgA

    Lane 6: 100 ng of human IgM

    Lane 7: 100 ng of human IgE

    Lane 8: 100 ng of rat IgG1

    Result: K16244_4E11 can specifically detect human IgG3 by Western blotting.

  • Cross-reactivity

    Microtiter wells were coated with human immunoglobulins with various isotypes. K16244_4E11 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.

    Result: K16244_4E11 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IgG3 Antibody [K16244_4E11]

  • Antibody specificity

    Human IgG3

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG1

  • Immunogen

    Recombinant human IgG3

  • Cross-reactivity

    K16244_4E11 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

  • Purification

    Protein A purified from cell culture supernatants


Full details

Recommend concentration


  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL


The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Immunoglobulin heavy constant gamma 3

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)