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Anti-Human IgG Antibody [KT48]

Catalog No. K11003R08A10C
  • Sandwich ELISA

    Microtiter wells were coated with KT48 at 5 µg/mL as the capture antibody. Human IgG was used as the antigen. Peroxidase conjugated mouse anti-human IgG monoclonal antibody (KT47) was used as the detection antibody.

    Result: KT48 and KT47 can be used as a matched antibody pair to detect and quantify the concentration of human IgG. 

  • Immunohistochemistry

    IHC-P analysis of human tonsil tissue by anti-human IgG antibody (KT48).

    IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human IgG primary antibody (KT48) at 0.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG (which cross reacts with rat IgG) was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with secondary antibody was used as background control.

    Result: Germinal center cells and squamous epithelial cells are positively stained at the cytoplasm (Only positively stained squamous epithelial cells are shown here).

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IgG Antibody [KT48]

  • Antibody specificity

    Human IgG1, IgG2, IgG3 and IgG4

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Rat / IgG2a

  • Immunogen

    A fusion protein contains human IgG Fc

  • Cross-reactivity

    Not tested

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

  • Purification

    Protein G purified from cell culture supernatants


Full details

Recommend concentration


  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    5 µg/mL

    KT48 can pair with peroxidase conjugated KT47 for sandwich ELISA. KT48 is used as the capture antibody.

  • Immunohistochemistry-paraffin (IHC-P)

    0.5 µg/mL


The applications above have already been verified. The antibody may be suitable for additional applications. 

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    KT48 may pair with KT47 for sandwich based immune assays.