Anti-Human IL2 Antibody [K16225_7E2]
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Sandwich ELISA
Microtiter wells were coated with K16225_7E2 at 3 µg/mL as the capture antibody. IL2 was used as the antigen. Peroxidase conjugated mouse anti-human IL2 monoclonal antibody (K03004_13E9) was used as the detection antibody.
Result: K16225_7E2 and K03004_13E9 can be used as a matched antibody pair to detect and quantify the concentration of IL2.
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Full details
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Product name
Anti-Human IL2 Antibody [K16225_7E2]
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Antibody specificity
Interleukin-2 (IL-2)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K16225_7E2
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Host / isotype
Mouse / IgG2b
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Immunogen
Recombinant human IL-2
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Cross reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Not tested
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Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
K16225_7E2 can pair with peroxidase conjugated K03004_13E9 for sandwich ELISA. K16225_7E2 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interleukin 2
Gene name:
IL2
Uniprot Accession:
P60568
Organism:
Homo sapiens (Human)
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Paired antibody information
K16225_7E2 may pair with K03004_13E9 for sandwich based immune assays.
