Microtiter wells were coated with K16225_7E2 at 3 µg/mL as the capture antibody. IL2 was used as the antigen. Peroxidase conjugated mouse anti-human IL2 monoclonal antibody (K03004_13E9) was used as the detection antibody.
Result: K16225_7E2 and K03004_13E9 can be used as a matched antibody pair to detect and quantify the concentration of IL2.
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Anti-Human IL2 Antibody [K16225_7E2]
Host / isotype
Mouse / IgG2b
Recombinant human IL-2
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
K16225_7E2 can pair with peroxidase conjugated K03004_13E9 for sandwich ELISA. K16225_7E2 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)
Paired antibody information
K16225_7E2 may pair with K03004_13E9 for sandwich based immune assays.