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Anti-Human CCL3 Antibody [K24010_9D3]

Catalog No. K24010M09D03C
  • Immunohistochemistry

    IHC-P analysis of human tonsil tissue by anti-human CCL3 antibody (K24010_9D3). 

    IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human CCL3 primary antibody (K24010_9D3) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. 

    Result: Germinal center cells and partial non- germinal center cells are positively stained at the cytoplasm and cell membrane.



Associated Products
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Overview

Full details

  • Product name

    Anti-Human CCL3 Antibody [K24010_9D3]

  • Antibody specificity

    C-C motif chemokine ligand 3

  • Species reactivity

    Human

  • Clonality

    Monoclonal

  • Clone number

    K24010_9D3

  • Host / isotype

    Mouse / IgG2b

  • Immunogen

    Recombinant human CCL3

  • Cross reactivity

    Not tested

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Purification

    Protein A purified from cell culture supernatants

  • Form

    Liquid

  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation

    Unconjugated

  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

Applications

Full details

Recommend concentration

Note

  • Indirect ELISA

    1 µg/mL

  • Immunohistochemistry-paraffin (IHC-P)

    10 µg/mL

Note

The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      C-C motif chemokine ligand 3

    • Gene name:

      CCL3

    • Uniprot Accession:

      P10147

    • Organism:

      Homo sapiens (Human)