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Anti-Human IL1A Antibody [K24011_18D3]

Catalog No. K24011M18D03C
  • Sandwich ELISA

    Microtiter wells were coated with K24011_18D3 at 3 µg/mL as the capture antibody. IL1A (Cat. P00084PC) was used as the antigen. Peroxidase conjugated mouse anti-human IL1A monoclonal antibody (K24011_17E10) was used as the detection antibody. 

    Result: K24011_18D3 and K24011_17E10 can be used as a matched antibody pair to detect and quantify the concentration of IL1A.

  • Immunoprecipitation

    Immunoprecipitation was performed by incubation of 2.5 µg K24011_18D3 with PMA and LPS treated THP-1 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-human IL1A (K24011_12H10) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. 

    Lane 1: PMA and LPS treated THP-1 lysate 

    Lane 2: Human IL1A immunoprecipitated from PMA and LPS treated THP-1 lysate by K24011_18D3 

    Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody 

    Result: K24011_18D3 can immunoprecipitate human IL1A.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IL1A Antibody [K24011_18D3]

  • Antibody specificity

    Interleukin 1 alpha (IL-1 alpha)

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG2b

  • Immunogen

    Recombinant human IL1A

  • Cross reactivity

    Not tested

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Purification

    Protein A purified from cell culture supernatants

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C


Full details

Recommend concentration


  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    K24011_18D3 can pair with peroxidase conjugated K24011_17E10 for sandwich ELISA. K24011_18D3 is used as the capture antibody.

  • Immunoprecipitation (IP)

    10 µg/mL


The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Interleukin 1 alpha

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    K24011_18D3 may pair with K24011_17E10 for sandwich based immune assays.