Microtiter wells were coated with K24011_18D3 at 3 µg/mL as the capture antibody. IL1A (Cat. P00084PC) was used as the antigen. Peroxidase conjugated mouse anti-human IL1A monoclonal antibody (K24011_17E10) was used as the detection antibody.
Result: K24011_18D3 and K24011_17E10 can be used as a matched antibody pair to detect and quantify the concentration of IL1A.
Immunoprecipitation was performed by incubation of 2.5 µg K24011_18D3 with PMA and LPS treated THP-1 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-human IL1A (K24011_12H10) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82.
Lane 1: PMA and LPS treated THP-1 lysate
Lane 2: Human IL1A immunoprecipitated from PMA and LPS treated THP-1 lysate by K24011_18D3
Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody
Result: K24011_18D3 can immunoprecipitate human IL1A.
There are no related products
Anti-Human IL1A Antibody [K24011_18D3]
Interleukin 1 alpha (IL-1 alpha)
Host / isotype
Mouse / IgG2b
Recombinant human IL1A
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
K24011_18D3 can pair with peroxidase conjugated K24011_17E10 for sandwich ELISA. K24011_18D3 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Interleukin 1 alpha
Homo sapiens (Human)
Paired antibody information
K24011_18D3 may pair with K24011_17E10 for sandwich based immune assays.