Anti-Human IL1A Antibody [K24011_17E10]
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Sandwich ELISA
Microtiter wells were coated with K24011_17E10 at 3 µg/mL as the capture antibody. IL1A (Cat. P00084PC) was used as the antigen. Peroxidase conjugated mouse anti-human IL1A monoclonal antibody (K24011_15A2) was used as the detection antibody.
Result: K24011_17E10 and K24011_15A2 can be used as a matched antibody pair to detect and quantify the concentration of IL1A.
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Full details
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Product name
Anti-Human IL1A Antibody [K24011_17E10]
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Antibody specificity
Interleukin 1 alpha (IL-1 alpha)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K24011_17E10
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Host / isotype
Mouse / IgG1
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Immunogen
Recombinant human IL1A
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Cross reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Not tested
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Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
K24011_17E10 can pair with peroxidase conjugated K24011_15A2 for sandwich ELISA. K24011_17E10 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interleukin 1 alpha
Gene name:
IL1A
Uniprot Accession:
P01583
Organism:
Homo sapiens (Human)
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Paired antibody information
K24011_17E10 may pair with K24011_15A2 for sandwich based immune assays.
