15 µg of U-2 OS lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K94016_16G4 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL18 band was visualized using ECL Western Blotting Substrate.
Result: K94016_16G4 can detect human IL18 by Western blotting.
Immunoprecipitation was performed by incubation of 2.5 µg K94016_16G4 with U-2 OS lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-human IL18 (K94016_13H1) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82.
Lane 1: U-2 OS lysate
Lane 2: Human IL18 immunoprecipitated from U-2 OS lysate by K94016_16G4
Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody
Result: K94016_16G4 can immunoprecipitate human IL18.
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Anti-Human IL18 Antibody [K94016_16G4]
Interleukin 18 (IL-18)
Host / isotype
Mouse / IgG1
Recombinant human IL18
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)
Paired antibody information
K94016_16G4 may pair with K94016_1C3, K94016_20H1 and K94016_10G5 for sandwich based immune assays.