25 ng of recombinant human IL3 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K92005_17F10 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IL3 band was visualized using ECL Western Blotting Substrate.
Result: K92005_17F10 can detect human IL3 by Western blotting.
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Anti-Human IL3 Antibody [K92005_17F10]
Interleukin 3 (IL-3)
Host / isotype
Mouse / IgG1
Recombinant human IL3
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)