Anti-Human IFNA2 Antibody [K29019_13B11]
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Western blotting
25 ng of recombinant human IFNA2 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K29019_13B11 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IFNA2 band was visualized using ECL Western Blotting Substrate.
Result: K29019_13B11 can detect recombinant human IFNA2 by Western blotting.
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Sandwich ELISA
Microtiter wells were coated with K29019_13B11 at 3 µg/mL as the capture antibody. IFNA2 (Cat. P00082PB) was used as the antigen. Peroxidase conjugated mouse anti- human IFNA2 monoclonal antibody (K29019_16A5) was used as the detection antibody.
Result: K29019_13B11 and K29019_16A5 can be used as a matched antibody pair to detect and quantify the concentration of IFNA2.
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Full details
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Product name
Anti-Human IFNA2 Antibody [K29019_13B11]
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Antibody specificity
Interferon alpha-2 (IFN-alpha-2, IFNA2)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K29019_13B11
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Host / isotype
Mouse / IgG1
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Immunogen
Recombinant human IFNA2
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Cross reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 5.59x 105 M-1s-1
Dissociation rate constant (koff): 3.48x 10-3 s-1
Equilibrium dissociation constant (KD): 6.22x 10-9 M -
Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Western blot (WB)
1 µg/mL
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
K29019_13B11 can pair with peroxidase conjugated K29019_16A5 for sandwich ELISA. K29019_13B11 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interferon alpha 2
Gene name:
IFNA2
Uniprot Accession:
P01563
Organism:
Homo sapiens (Human)
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Paired antibody information
K29019_13B11 may pair with K29019_16A5, K29019_20D10, K29019_9C6, K29019_12F8, K03003_1B2 and KT107 for sandwich based immune assays.
