Anti-Human IFNA1 Antibody [K92003_1D8]
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Western blotting
25 ng of recombinant human IFNA1 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K92003_1D8 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IFNA1 band was visualized using ECL Western Blotting Substrate.
Result: K92003_1D8 can detect human IFNA1 by Western blotting.
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Sandwich ELISA
Microtiter wells were coated with K92003_1D8 at 3 µg/mL as the capture antibody. IFNA1 (Cat. P00703PB) was used as the antigen. Peroxidase conjugated mouse anti-human IFNA1 monoclonal antibody (K92003_15C12) was used as the detection antibody.
Result: K92003_1D8 and K92003_15C12 can be used as a matched antibody pair to detect and quantify the concentration of IFNA1.
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Full details
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Product name
Anti-Human IFNA1 Antibody [K92003_1D8]
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Antibody specificity
Interferon alpha 1 (IFN-alpha-1)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K92003_1D8
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Host / isotype
Mouse / IgG2a
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Immunogen
Recombinant human IFNA1
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Cross reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 1.80x 106 M-1s-1
Dissociation rate constant (koff): 1.01x 10-3 s-1
Equilibrium dissociation constant (KD): 5.63x 10-10 M -
Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Western blot (WB)
1 µg/mL
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
K92003_1D8 can pair with peroxidase conjugated K92003_15C12 for sandwich ELISA. K92003_1D8 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interferon alpha 1
Gene name:
IFNA1
Uniprot Accession:
P01562
Organism:
Homo sapiens (Human)
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Paired antibody information
K92003_1D8 may pair with K92003_13B4, K92003_3A1, K92007_9F10, K92007_6H2, K92007_9H4 and K92007_1C3 for sandwich based immune assays.
