25 ng of recombinant human IL15 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K94008_10D2 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL15 band was visualized using ECL Western Blotting Substrate.
Result: K94008_10D2 can detect human IL15 by Western blotting.
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Anti-Human IL15 Antibody [K94008_10D2]
Host / isotype
Mouse / IgG2b
Recombinant human IL-15
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)
Paired antibody information
K94008_10D2 may pair with K94008_7D11 for sandwich based immune assays.