Anti-Human TNF Antibody [K29020_5G7]

25 ng of recombinant human TNF was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K29020_5G7 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. TNF band was visualized using ECL Western Blotting Substrate. 

Result: K29020_5G7 can detect recombinant human TNF by Western blotting.

Immunoprecipitation was performed by incubation of 2.5 µg K29020_5G7 with PMA, LPS and BFA treated THP-1 lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-human TNF (K29020_9E6) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. 

Lane 1: Human TNF immunoprecipitated from PMA, LPS and BFA treated THP-1 lysate by K29020_5G7 

Lane 2: The same as Lane 2 but KT82 was used as IgG isotype control antibody 

Result: K29020_5G7 can immunoprecipitate human TNF.