25 ng of recombinant human TNF was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K29020_5G7 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. TNF band was visualized using ECL Western Blotting Substrate.
Result: K29020_5G7 can detect recombinant human TNF by Western blotting.
Immunoprecipitation was performed by incubation of 2.5 µg K29020_5G7 with PMA, LPS and BFA treated THP-1 lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-human TNF (K29020_9E6) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82.
Lane 1: Human TNF immunoprecipitated from PMA, LPS and BFA treated THP-1 lysate by K29020_5G7
Lane 2: The same as Lane 2 but KT82 was used as IgG isotype control antibody
Result: K29020_5G7 can immunoprecipitate human TNF.
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Anti-Human TNF Antibody [K29020_5G7]
Tumor necrosis factor (TNF-alpha
Host / isotype
Mouse / IgG2a
Recombinant human TNF
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Tumor necrosis factor
Homo sapiens (Human)
Paired antibody information
K29020_5G7 may pair with K29020_2H4 and K29020_9E6 for sandwich based immune assays.