Anti-Human EGF Antibody [KT2]
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Sandwich ELISA
Microtiter wells were coated with KT57 at 3 µg/mL as the capture antibody. EGF was used as the antigen. Peroxidase conjugated mouse anti-human EGF monoclonal antibody (KT2) was used as the detection antibody.
Result: KT2 and KT57 can be used as a matched antibody pair to detect and quantify the concentration of EGF.
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Full details
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Product name
Anti-Human EGF Antibody (HRP) [KT2]
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Antibody specificity
Pro-epidermal growth factor (EGF)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
KT2
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Host / isotype
Mouse / IgG2a
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Immunogen
Recombinant human EGF
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Cross reactivity
Not tested
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.01% preservative
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Conjugation
Conjugated with peroxidase
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Sandwich ELISA
1 µg/mL
Peroxidase conjugated KT2 can pair with KT57 for sandwich ELISA. KT57 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Epidermal growth factor
Gene name:
EGF
Uniprot Accession:
P01133
Organism:
Homo sapiens (Human)
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Paired antibody information
KT2 may pair with KT57 for sandwich based immune assays.
