Microtiter wells were coated with KT57 at 3 µg/mL as the capture antibody. EGF was used as the antigen. Peroxidase conjugated mouse anti-human EGF monoclonal antibody (KT2) was used as the detection antibody.
Result: KT2 and KT57 can be used as a matched antibody pair to detect and quantify the concentration of EGF.
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Anti-Human EGF Antibody (HRP) [KT2]
Pro-epidermal growth factor (EGF)
Host / isotype
Mouse / IgG2a
Recombinant human EGF
1 mg/mL in phosphate buffered saline containing 0.01% preservative
Conjugated with peroxidase
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Peroxidase conjugated KT2 can pair with KT57 for sandwich ELISA. KT57 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Epidermal growth factor
Homo sapiens (Human)
Paired antibody information
KT2 may pair with KT57 for sandwich based immune assays.