25 ng of recombinant human IFNA1 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K92003_13C1 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IFNA1 band was visualized using ECL Western Blotting Substrate.
Result: K92003_13C1 can detect human IFNA1 by Western blotting.
Microtiter wells were coated with K92003_13C1 at 3 µg/mL as the capture antibody. IFNA1 (Cat. P00703PB) was used as the antigen. Peroxidase conjugated mouse anti- human IFNA1 monoclonal antibody (K06251_12D6) was used as the detection antibody.
Result: K92003_13C1 and K06251_12D6 can be used as a matched antibody pair to detect and quantify the concentration of human IFNA1.
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Anti-Human IFNA1 Antibody [K92003_13C1]
Interferon alpha 1 (IFN-alpha-1)
Host / isotype
Mouse / IgG2b
Recombinant human IFNA1
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 2.29x 106 M-1s-1
Dissociation rate constant (koff): 1.14x 10-3 s-1
Equilibrium dissociation constant (KD): 4.98x 10-10 M
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
K92003_13C1 can pair with peroxidase conjugated K06251_12D6 for sandwich ELISA. K92003_13C1 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Interferon alpha 1
Homo sapiens (Human)
Paired antibody information
K92003_13C1 may pair with K92003_3A12, K92003_7A12 ,K92003_11H8, K92003_13B1, K92003_13B4, K92003_13E6, K92007_13G2, K92003_15B12 and K92007_12D6 for sandwich based immune assays.