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Anti-Human IL12A Antibody [K92010_16E1]

Catalog No. K92010U16E01C
  • Western blotting

    25 ng of recombinant human IL12A and 25 ng of recombinant human IL12B were run on 6- 18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K92010_16E1 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL12A band was visualized using ECL Western Blotting Substrate. 

    Result: K92010_16E1 can detect human IL12A by Western blotting.

  • Sandwich ELISA

    Microtiter wells were coated with K92010_16E1 at 3 µg/mL as the capture antibody. IL12A (Cat. P00727PB) was used as the antigen. Peroxidase conjugated mouse anti-human IL12A monoclonal antibody (K92010_6H3) was used as the detection antibody. 

    Result: K92010_16E1 and K92010_6H3 can be used as a matched antibody pair to detect and quantify the concentration of IL12A.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IL12A Antibody [K92010_1E2]

  • Antibody specificity

    Interleukin-12A (IL-12A)

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse IgG with unknown isotype

  • Immunogen

    Recombinant human IL-12A

  • Cross reactivity

    Not tested

  • Kinetic characterization by BLI (biolayer interferometry)

    Not tested

  • Purification

    Protein A purified from cell culture supernatants

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C


Full details

Recommend concentration


  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    K92010_16E1 can pair with peroxidase conjugated K92010_6H3 for sandwich ELISA. K92010_16E1 is used as the capture antibody.


The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Interleukin 12A

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    K92010_16E1 may pair with K92010_6H3 for sandwich based immune assays.