Anti-Human IL12A Antibody [K92010_1E2]
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Western blotting
25 ng of recombinant human IL12A and 25 ng of recombinant human IL12B were run on 6- 18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K92010_1E2 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL12A band was visualized using ECL Western Blotting Substrate.
Result: K92010_1E2 can detect human IL12A by Western blotting.
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Full details
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Product name
Anti-Human IL12A Antibody [K92010_1E2]
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Antibody specificity
Interleukin-12A (IL-12A)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K92010_1E2
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Host / isotype
Mouse IgG with unknown isotype
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Immunogen
Recombinant human IL-12A
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Cross reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Not tested
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Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Western blot (WB)
1 µg/mL
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Indirect ELISA
1 µg/mL
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interleukin 12A
Gene name:
IL12A
Uniprot Accession:
P29459
Organism:
Homo sapiens (Human)
