25 ng of recombinant human IL12A and 25 ng of recombinant human IL12B were run on 6- 18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K92010_3D8 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL12A band was visualized using ECL Western Blotting Substrate.
Result: K92010_3D8 can detect human IL12A by Western blotting.
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Anti-Human IL12A Antibody [K92010_3D8]
Host / isotype
Mouse / IgG3
Recombinant human IL-12A
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)