25 ng of recombinant human IL-6 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K06289_1E11 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IL-6 band was visualized using ECL Western Blotting Substrate.
Result: K06289_1E11 can detect recombinant human IL-6 by Western blotting.
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Anti-Human IL6 Antibody [K06289_1E11]
Host / isotype
Mouse / IgG2a
Recombinant human IL-6
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 3.00x 104 M-1s-1
Dissociation rate constant (koff): 7.81x 10-4 s-1
Equilibrium dissociation constant (KD): 2.60x 10-8 M
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)