Anti-Human CCL2 Antibody [K06288_16E11]
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Sandwich ELISA
Microtiter wells were coated with K06288_16E11 at 3 µg/mL as the capture antibody. CCL2 was used as the antigen. Peroxidase conjugated mouse anti-human CCL2 monoclonal antibody (K06288_1C2) was used as the detection antibody.
Result: K06288_16E11 and K06288_1C2 can be used as a matched antibody pair to detect and quantify the concentration of CCL2.
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Full details
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Product name
Anti-Human CCL2 Antibody [K06288_16E11]
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Antibody specificity
C-C motif chemokine ligand 2 (MCP1)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K06288_16E11
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Host / isotype
Mouse / IgG2b
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Immunogen
Recombinant human CCL2
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Cross reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Not tested
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Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
K06288_16E11 can pair with peroxidase conjugated K06288_1C2 for sandwich ELISA. K06288_16E11 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
C-C motif chemokine ligand 2
Gene name:
CCL2
Uniprot Accession:
P13500
Organism:
Homo sapiens (Human)
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Paired antibody information
K06288_16E11 may pair with K06288_1C2, K06288_4G5, KT221 and K06288_16E11 for sandwich based immune assays.
