25 ng of recombinant human TNF was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K03008_3A9 at 1 µg/mL was used as primary antibody and peroxidase conjugated goat anti-rat IgG was used as secondary antibody. TNF-alpha band was visualized using ECL Western Blotting Substrate.
Result: K03008_3A9 can detect recombinant human TNF-alpha by Western blotting.
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Anti-Human TNF Antibody [K03008_3A9]
Tumour necrosis factor (TNF-alpha, TNFα)
Host / isotype
Rat / IgG2a
Recombinant human TNF
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon):1.76x 105 M-1s-1
Dissociation rate constant (koff):1.39x 10-4 s-1
Equilibrium dissociation constant (KD): 7.09x 10-10 M
Protein G purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Tumor necrosis factor
Homo sapiens (Human)