Anti-Human IL2 Antibody [K16225_2H10]
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Western blotting
25 ng of recombinant human IL-2 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose paper. K16225_2H10 at 1 µg/mL was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. IL-2 band was visualized using ECL Western Blotting Substrate.
Result: K16225_2H10 can detect recombinant human IL-2 by Western blotting.
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Sandwich ELISA
Microtiter wells were coated with K16225_2H10 at 3 µg/mL as the capture antibody. IL-2 was used as the antigen. Peroxidase conjugated mouse anti-human IL-2 monoclonal antibody (KT7) was used as the detection antibody.
Result: K16225_2H10 and KT7 can be used as a matched antibody pair to detect and quantify the concentration of IL-2.
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Cross reactivity
Microtiter wells were coated with human cytokines. K16225_2H10 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody.
Results: K16225_2H10 does not cross-react with human IL1B, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Full details
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Product name
Anti-Human IL2 Antibody [K16225_2H10]
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Antibody specificity
Interleukin-2 (IL-2, IL2)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K16225_2H10
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Host / isotype
Mouse / IgG1
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Immunogen
Recombinant human IL-2
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Cross reactivity
K16225_2H10 does not cross-react with human IL1B, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 2.55x 104 M-1s-1
Dissociation rate constant (koff): 7.56x 10-4 s-1
Equilibrium dissociation constant (KD): 2.96x 10-8 M -
Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Western blot (WB)
1 µg/mL
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
K16225_2H10 can pair with peroxidase conjugated KT7 for sandwich ELISA. K16225_2H10 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interleukin 2
Gene name:
IL2
Uniprot Accession:
P60568
Organism:
Homo sapiens (Human)
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Paired antibody information
K16225_2H10 may pair with K24002_3G1, K24002_7E2, K24002_9A12, K24002_12G6, KT220, KT7 and K16225_5H10 for sandwich based immune assays.
