25 ng of recombinant human IL-6 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K06301_22C3 at 1 µg/mL was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. IL-6 band was visualized using ECL Western Blotting Substrate.
Result: K06301_22C3 can detect recombinant human IL-6 by Western blotting.
Microtiter wells were coated with K06301_22C3 at 3 µg/mL as the capture antibody. Peroxidase conjugated mouse anti-human IL-6 monoclonal antibody (K16255_5H11) was used as the detection antibody.
Result: K06301_22C3 and K16255_5H11 can be used as matched antibody pairs to detect and quantify the concentration of human IL-6.
Microtiter wells were coated with various human cytokines. K06301_22C3 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K06301_22C3 does not cross-react with human IL1B, IL2, IL4, IL5, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Anti-Human IL6 Antibody [K06301_22C3]
Host / isotype
Mouse / IgG2b
Recombinant human IL-6
Not cross-react with human IL1B, IL2, IL4, IL5, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 2.37x 106 M-1s-1
Dissociation rate constant (koff):2.81x 10-4 s-1
Equilibrium dissociation constant (KD): 1.18x 10-10 M
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
K06301_22C3 can pair with peroxidase conjugated K16255_5H11 for sandwich ELISA. K06301_22C3 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)
Paired antibody information
K06301_22C3 may pair with K16255_5H11, KAA209_6A12, K06301_19H9, K03005_15B1 and KT105 for sandwich based immune assays.