Anti-Human IL6 Antibody [K06301_22G12]
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Western blotting
25 ng of recombinant human IL-6 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K06301_22G12 at 1 µg/mL was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. IL-6 band was visualized using ECL Western Blotting Substrate.
Result: K06301_22G12 can detect recombinant human IL-6 by Western blotting.
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Sandwich ELISA
Microtiter wells were coated with K06301_22G12 at 3 µg/mL as the capture antibody. Peroxidase conjugated mouse anti-human IL-6 monoclonal antibody (K03005_15B1) was used as the detection antibody.
Result: K06301_22G12 and K03005_15B1 can be used as matched antibody pairs to detect and quantify the concentration of human IL-6.
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Cross reactivity
Microtiter wells were coated with various human cytokines. K06301_22G12 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K06301_22G12 does not cross-react with human IL1B, IL2, IL4, IL5, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Full details
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Product name
Anti-Human IL6 Antibody [K06301_22G12]
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Antibody specificity
Interleukin-6 (IL-6)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K06301_22G12
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Host / isotype
Mouse / IgG1
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Immunogen
Recombinant human IL-6
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Cross reactivity
Not cross-react with human IL1B, IL2, IL4, IL5, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF
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Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 1.76x 106 M-1s-1
Dissociation rate constant (koff): 1.07x 10-3 s-1
Equilibrium dissociation constant (KD): 6.09x 10-10 M -
Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Indirect ELISA
1 µg/mL
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Western blot (WB)
1 µg/mL
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Sandwich ELISA
3 µg/mL
K06301_22G12 can pair with peroxidase conjugated K03005_15B1 for sandwich ELISA. K06301_22G12 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interleukin 6
Gene name:
IL6
Uniprot Accession:
P05231
Organism:
Homo sapiens (Human)
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Paired antibody information
K06301_22G12 may pair with K03005_15B1 and K06301_19H9 for sandwich based immune assays.
