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Anti-Human IL6 Antibody [K16255_5H11]

Catalog No. K16255M05H11C
  • Western blotting

    25 ng of recombinant human IL-6 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K16255_5H11 at 1 µg/mL was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. IL-6 band was visualized using ECL Western Blotting Substrate. 

    Result: K16255_5H11 can detect recombinant human IL-6 by Western blotting.


  • Sandwich ELISA

    Microtiter wells were coated with K16255_5H11 at 3 µg/mL as the capture antibody. Peroxidase conjugated mouse anti-human IL-6 monoclonal antibody (K06301_19H9) was used as the detection antibody. 

    Result: K16255_5H11 and K06301_19H9 can be used as matched antibody pairs to detect and quantify the concentration of human IL-6.

  • Cross reactivity

    Microtiter wells were coated with various human cytokines. K16255_5H11 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

    Result: K06301_5H11 does not cross-react with human IL1B, IL2, IL4, IL5, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.

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Overview

Full details

  • Product name

    Anti-Human IL6 Antibody [K16255_5H11]

  • Antibody specificity

    Interleukin-6 (IL-6)

  • Species reactivity

    Human

  • Clonality

    Monoclonal

  • Clone number

    K16255_5H11

  • Host / isotype

    Mouse / IgG2b

  • Immunogen

    Recombinant human IL-6

  • Cross reactivity

    Not cross-react with human IL1B, IL2, IL4, IL5, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon): 1.75x 106 M-1s-1
    Dissociation rate constant (koff): 2.52x 10-3 s-1
    Equilibrium dissociation constant (KD): 1.44x 10-9 M

  • Purification

    Protein A purified from cell culture supernatants

  • Form

    Liquid

  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation

    Unconjugated

  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

Applications

Full details

Recommend concentration

Note

  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    K16255_5H11 can pair with peroxidase conjugated K06301_19H9 for sandwich ELISA. K16255_5H11 is used as the capture antibody.

Note

The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen

    • Protein name:

      Interleukin 6

    • Gene name:

      IL6

    • Uniprot Accession:

      P05231

    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    K16255_5H11 may pair with K06301_19H9, K06301_22C3, K06301_16H9,K03005_15B1, K06301_13E2 and KT105 for sandwich based immune assays.