Anti-Human IL6 Antibody [K06301_19H9]

25 ng of recombinant human IL-6 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K06301_19H9 at 1 µg/mL was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. IL-6 band was visualized using ECL Western Blotting Substrate.

Result: K06301_19H9 can detect recombinant human IL-6 by Western blotting

Microtiter wells were coated with K06301_19H9 at 3 µg/mL as the capture antibody. Peroxidase conjugated mouse anti-human IL-6 monoclonal antibody (K16255_5H11) was used as the detection antibody. 

Result: K06301_19H9 and K16255_5H11 can be used as matched antibody pairs to detect and quantify the concentration of human IL-6.

Microtiter wells were coated with various human cytokines. K06301_19H9 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

Result: K06301_19H9 does not cross-react with human IL1B, IL2, IL4, IL5, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.