25 ng of recombinant human IL-2 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K16225_7A7 at 1 µg/mL was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. IL-2 band was visualized using ECL Western Blotting Substrate.
Result: K16225_7A7 can detect recombinant human IL-2 by Western blotting.
Microtiter wells were coated with various human cytokines. K16225_7A7 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K16225_7A7 does not cross-react with human IL1B, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, IL17A, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
There are no related products
Anti-Human IL2 Antibody [K16225_7A7]
Host / isotype
Mouse / IgG1
Recombinant human IL-2
Not cross-react with human IL1B, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, IL17A, CSF2, IFNA, IFNG, CCL2, KITLG and TNF
Kinetic characterization by BLI (biolayer interferometry)
Protein A purified from cell culture supernatants
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)