Anti-Human IL2 Antibody [K16225_6D2]

Catalog No. K16225W06D02C

Western blotting

25 ng of recombinant human IL-2 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K16225_6D2 at 1 µg/mL was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. IL-2 band was visualized using ECL Western Blotting Substrate.
Result: K16225_6D2 can detect recombinant human IL-2 by Western blotting.
Sandwich ELISA

Microtiter wells were coated with K16225_6D2 at 3 µg/mL as the capture antibody. Peroxidase conjugated mouse anti-human IL-2 monoclonal antibody (K16225_4H5) was used as the detection antibody.
Result: K16225_6D2 and K16225_4H5 can be used as matched antibody pairs to detect and quantify the concentration of IL-2.
Cross reactivity

Microtiter wells were coated with various human cytokines. K16225_6D2 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K16225_6D2 does not cross-react with human IL4, IL5, IL6, IL7, CXCL8, IL1B, IL9, IL10, IL11, IL12, IL13, IL17A, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.

Associated Products

Download

Overview

Full details

Product name

Anti-Human IL2 Antibody [K16225_6D2]
Antibody specificity

Interleukin-2 (IL-2)
Species reactivity

Human
Clonality

Monoclonal
Clone number

K16225_6D2
Host / isotype

Mouse / IgG2b
Immunogen

Recombinant human IL-2
Cross reactivity

Not cross-react with human IL4, IL5, IL6, IL7, CXCL8, IL1B, IL9, IL10, IL11, IL12, IL13, IL17A, CSF2, IFNA, IFNG, CCL2, KITLG and TNF
Kinetic characterization by BLI (biolayer interferometry)

Not tested
Purification

Protein A purified from cell culture supernatants
Form

Liquid
Concentration

1 mg/mL in phosphate buffered saline containing 0.09% preservative
Conjugation

Unconjugated
Shipping, storage and shelf life

Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C

Applications

Full details

Recommend concentration

Note

Western blot (WB)

1 µg/mL
Indirect ELISA

1 µg/mL
Sandwich ELISA

3 µg/mL

K16225_6D2 can pair with peroxidase conjugated K16225_4H5 for sandwich ELISA. K16225_6D2 is used as the capture antibody.

Note

The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information