25ng of recombinant human IFNG was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K29001_11F4 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IFNG band was visualized using ECL Western Blotting Substrate.
Result: K29001_11F4 can detect recombinant human IFNG by Western blotting.
Microtiter wells were coated with K29001_11F4 at 3 µg/mL as the capture antibody. Recombinant human IFNG (Cat. P00705PA) was used as the antigen. Peroxidase conjugated mouse anti-human IFNG monoclonal antibody (KT218) was used as the detection antibody.
Result: K29001_11F4 and KT218 can be used as a matched antibody pair to detect and quantify the concentration of IFNG.
Microtiter wells were coated with various human cytokines. K29001_11F4 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K29001_11F4 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, CCL2, KITLG and TNF.
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Anti-Human IFNG Antibody [K29001_11F4]
Host / isotype
Mouse / IgG2b
Recombinant human IFNG (P00705PA)
K29001_11F4 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, CCL2, KITLG and TNF.
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon):6.41x 105 M-1s-1
Dissociation rate constant (koff):1.16x 10-3 s-1
Equilibrium dissociation constant (KD): 1.81x 10-9 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
Western blot (WB)
K29001_11F4 can pair with peroxidase conjugated KT218 for sandwich ELISA. K29001_11F4 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Homo sapiens (Human)
Paired antibody information
K29001_11F4 may pair with KT218, K29001_12E1, K29001_5C7, K29001_1C9 and K29001_1D8 for sandwich based immune assays.