Your privacy is very important to us.When you visit our website,please agree to the use of all cookies.For more information about personal data processing,please go to Privacy.

中

Anti-Human IFNG Antibody [K29001_11F4]

Catalog No. K29001U11F04C
  • Western blotting

    25ng of recombinant human IFNG was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K29001_11F4 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IFNG band was visualized using ECL Western Blotting Substrate.

    Result: K29001_11F4 can detect recombinant human IFNG by Western blotting.



  • Sandwich ELISA

    Microtiter wells were coated with K29001_11F4 at 3 µg/mL as the capture antibody. Recombinant human IFNG (Cat. P00705PA) was used as the antigen. Peroxidase conjugated mouse anti-human IFNG monoclonal antibody (KT218) was used as the detection antibody. 

    Result: K29001_11F4 and KT218 can be used as a matched antibody pair to detect and quantify the concentration of IFNG.

  • Cross reactivity

    Microtiter wells were coated with various human cytokines. K29001_11F4 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.

    Result: K29001_11F4 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, CCL2, KITLG and TNF.

Associated Products

Download

  • There are no related products

Overview

Full details

  • Product name

    Anti-Human IFNG Antibody [K29001_11F4]

  • Antibody specificity

    Interferon gamma

  • Species reactivity

    Human

  • Clonality

    Monoclonal

  • Clone number

    K29001_11F4

  • Host / isotype

    Mouse / IgG2b

  • Immunogen

    Recombinant human IFNG (P00705PA)

  • Cross-reactivity

    K29001_11F4 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, CCL2, KITLG and TNF.

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon):6.41x 105 M-1s-1
    Dissociation rate constant (koff):1.16x 10-3 s-1
    Equilibrium dissociation constant (KD): 1.81x 10-9 M

  • Form

    Liquid

  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation

    Unconjugated

  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

  • Purification

    Protein A purified from cell culture supernatants

Applications

Full details

Recommend concentration

Note

  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    K29001_11F4 can pair with peroxidase conjugated KT218 for sandwich ELISA. K29001_11F4 is used as the capture antibody.

Note

The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen

    • Protein name:

      Interferon gamma

    • Gene name:

      IFNG

    • Uniprot Accession:

      P01579

    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    K29001_11F4 may pair with KT218, K29001_12E1, K29001_5C7, K29001_1C9 and K29001_1D8 for sandwich based immune assays.