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Anti-Human IFNG Antibody [K29001_1C9]

Catalog No. K29001U01C09C
  • Western blotting

    25 ng of recombinant human IFNG was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K29001_1C9 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IFNG band was visualized using ECL Western Blotting Substrate.

    Result: K29001_1C9 can detect recombinant human IFNG by Western blotting.

  • Sandwich ELISA

    Microtiter wells were coated with K29001_1C9 at 3 µg/mL as the capture antibody. Human IFNG was used as the antigen. Peroxidase conjugated mouse anti-human IFNG monoclonal antibody (K29001_11F4) was used as the detection antibody.

    Result: K29001_1C9 and K29001_11F4 can be used as a matched antibody pair to detect and quantify the concentration of IFNG. 

  • Cross-reactivity

    Microtiter wells were coated with various human cytokines. K29001_1C9 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.

    Result: K29001_1C9 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, CCL2, KITLG and TNF.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IFNG Antibody [K29001_1C9]

  • Antibody specificity

    Interferon gamma

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG2b

  • Immunogen

    Recombinant human interferon gamma (P00705PA)

  • Cross-reactivity

    K29001_1C9 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, CCL2, KITLG and TNF.

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon): 4.82x 105 M-1s-1
    Dissociation rate constant (koff):3.53 x 10-4 s-1
    Equilibrium dissociation constant (KD): 7.34x 10-10 M

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

  • Purification

    Protein A purified from cell culture supernatants


Full details

Recommend concentration


  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    K29001_1C9 can pair with peroxidase conjugated K29001_11F4 for sandwich ELISA. K29001_1C9 is used as the capture antibody.


The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Interferon gamma

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    K29001_1C9 may pair with K29001_1B9, K29001_11F4, K29001_5C7, K29001_10H3, KT218 and KT219 for sandwich based immune assays.