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Anti-Human IFNG Antibody [KT219]

Catalog No. K29001U06H04C
  • Western blotting

    25 ng of recombinant human IFNG was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT219 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IFNG band was visualized using ECL Western Blotting Substrate.

    Result: KT219 can detect recombinant human IFNG by Western blotting.

  • Sandwich ELISA

    Microtiter wells were coated with KT219 at 3 µg/mL as the capture antibodyHuman IFNG was used as the antigen. Peroxidase conjugated mouse anti-human IFNG monoclonal antibody (KT218) was used as the detection antibody.

    Result: KT219 and KT218 can be used as a matched antibody pair to detect and quantify the concentration of IFNG.

  • Cross reactivity

    Microtiter wells were coated with various human cytokines. KT219 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.

    Result: KT219 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, CCL2, KITLG and TNF.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IFNG Antibody [KT219]

  • Antibody specificity

    Interferon gamma

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG1

  • Immunogen

    Recombinant human interferon gamma (P00705PA)

  • Cross-reactivity

    KT219 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, CCL2, KITLG and TNF.

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon): 2.63x 105 M-1s-1
    Dissociation rate constant (koff): 3.39 x 10-4 s-1
    Equilibrium dissociation constant (KD): 1.29 x 10-9 M

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

  • Purification

    Protein A purified from cell culture supernatants


Full details

Recommend concentration


  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    KT219 can pair with peroxidase conjugated KT218 for sandwich ELISA. KT219 is used as the capture antibody.


The applications above have already been verified. The antibody may be suitable for additional applications.

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Interferon gamma

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    KT219 may pair with K29001_1C9, K29001_1D8, KT218, KT219 and K29001_11F4 for sandwich based immune assays.