25 ng of recombinant human IFNA2 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT107 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the secondary antibody. IFNA2 band was visualized using ECL Western Blotting Substrate.
Result: KT107 can detect recombinant human IFNA2 by Western blotting.
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Anti-Human IFNA2 Antibody [KT107]
Interferon alpha-2 (IFN-alpha-2, IFNα2, IFNA2)
Host / isotype
Rat / IgG2a
Recombinant human IFNA2b
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 7.15x 104 M-1s-1
Dissociation rate constant (koff): 2.35x 10-3 s-1
Equilibrium dissociation constant (KD):3.29x 10-8 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein G purified from cell culture supernatants
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Interferon alpha 2
Homo sapiens (Human)