Anti-Human CXCL8 Antibody [KT215]
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Western blotting
25 ng of recombinant human CXCL8 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT215 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. CXCL8 band was visualized using ECL Western Blotting Substrate.
Result: KT215 can detect human CXCL8 by Western blotting.
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Sandwich ELISA
Microtiter wells were coated with KT215 at 3 µg/mL as the capture antibody. CXCL8 was used as the antigen. Peroxidase conjugated mouse anti-human CXCL8 monoclonal antibody (KT212) was used as the detection antibody.
Result: KT215 and KT212 can be used as a matched antibody pair to detect and quantify the concentration of CXCL8.
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Cross reactivity
Microtiter wells were coated with various human cytokines. KT215 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: KT215 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Full details
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Product name
Anti-Human CXCL8 Antibody [KT215]
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Antibody specificity
C-X-C motif chemokine ligand 8 (Interleukin 8, IL-8)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
KT215
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Host / isotype
Mouse / IgG1
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Immunogen
Recombinant human CXCL8 (P00726PA)
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Cross-reactivity
KT215 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, IL9, IL10, IL11, IL12, IL13, IL17A, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon):1.14x 105 M-1s-1
Dissociation rate constant (koff):1.93x 10-3 s-1
Equilibrium dissociation constant (KD):1.68x 10-8 M -
Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80°C
Full details
Recommend concentration
Note
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Western blot (WB)
1 µg/mL
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
KT215 can pair with peroxidase conjugated KT212 for sandwich ELISA. KT215 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
C-X-C motif chemokine ligand 8
Gene name:
CXCL8
Uniprot Accession:
P10145
Organism:
Homo sapiens (Human)
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Paired antibody information
KT215 may pair with KT212, KT214 for sandwich based immune assays.
